A laboratory practice that uses the polymerase chain reaction‐sequence specific priming technique to rapidly screen for HLA‐DR2 allotype from germline DNA in …
LX Li, W Tian, J Zhang - Biochemistry and Molecular Biology …, 2020 - Wiley Online Library
In this article, we describe an in‐house polymerase chain reaction‐sequence specific
priming (PCR‐SSP) assay designed for undergraduate medical students as part of the
experimental pathogen biology and immunology (EPBI) course. It screens human leukocyte
antigen (HLA)‐DR2 allotype from genomic DNA samples using a rapid and single‐tube
PCR technique, yielding definitive typing result without conventional post‐amplification step
like probing or Sanger sequencing. This laboratory exercise offers the undergraduate …
priming (PCR‐SSP) assay designed for undergraduate medical students as part of the
experimental pathogen biology and immunology (EPBI) course. It screens human leukocyte
antigen (HLA)‐DR2 allotype from genomic DNA samples using a rapid and single‐tube
PCR technique, yielding definitive typing result without conventional post‐amplification step
like probing or Sanger sequencing. This laboratory exercise offers the undergraduate …